Part:BBa_M1208:Design

Kill gene intermediate - Holin/Autolysin portion

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1763
  Illegal AgeI site found at 1833
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 724
  

Design Notes

The sequence is regulated by a tet repressible promoter. This promoter was chosen because of the repressor half-life time and because it allows the user to abort the culture with the addition of tetracyline.